SAXS FrameSlice

Overview

Determine the limits of radiation-induced aggregation or damage, and adjust your data accordingly, particularly for the Gunier region of your data
Remove data points from either end of your data
Improve signal to noise ratios, particularly at the Wide-angle region of your data

The file extension must be .dat
The files should not have headers or footers
The file contents should consist of 3 columns in plain-text using space-delimited values from post-processed image data
The 3 columns should consist of the 'scattering angle q', 'intensity', and 'errors', respectively
Values in the columns should be numbers that are either floats or in exponential notation
The experimental setup should be the same across all files, ie. have the same scattering angles and have the same number of lines of data

Click the white upload box to get a menu dialog to upload your files or...
Drag and drop your files onto the white upload box
Chrome, Firefox, Opera, Vivaldi, and Brave users may drag-and-drop a directory folder directly onto the box to upload files

Always wait until the current job is finished (you see the graph) before uploading files to submit another job when using multiple windows from the same browser
After the files have uploaded and the graph appears, check that all of your files have been processed by looking at the number of files, and their names under the graph

Displays first and last data file, "test-0001.dat to test-0008.dat", that was processed to generate the data
If there was an error, a message will appear at this position

This graph displays three sets of data, in this example the "Line" format has been chosen from the "Chart" controls option:
1. Data corresponding to the first processed image (colored blue and labeled "Img 1" in the legend)
2. Data corresponding to all images (colored orange and labeled "Img 1-N Avg", where N is the total number of images) - here the angles are all the same (a requirement for this program) - but the intensities are averaged among all files that were uploaded
3. Data that you control (colored black and labeled "Dynamic") that represents the eventual output file that will be generated upon hitting the "Download" button
Upon loading, the "Dynamic" data will overlap with the "Img 1" data until the number of images to include using the data inputs is increased
The main graph has 3 shaded areas corresponding to different "regions" that may be independently represented by different numbers of image data to include in the average calculation:
1. The Gunier region(shaded green)
2. The Porod region(shaded yellow)
3. The Wide region(shaded red)
The x-axis of the graph shows the scattering angle
Zoom and Drag Features:
- Zoom: Hover your pointer over the graph, then use the click-wheel of your mouse to zoom in and out
- Drag: Click the graph and drag to the left of the right

The grey area depicts the relative region of the main upper graph that when zoomed in
If the chart is in line mode, a corresponding plot of the line is displayed in the zoom graph
Zoom and Drag Features:
- Drag: When the main upper graph is zoomed in, you may click the lower zoom graph and drag to the left of the right to move the main upper graph

Focus and Hide features:
- Focus: To show just one particular graph, hover your pointer over the respective title in legend
- Hide/Unhide: To hide/unhide a graph, click the respective title in the legend

When the user input parameters are in "Slider" mode, a slider bar is shown below the graphs
This slider controls the boundaries of the different regions of the graph again colors correspond to:
1. The Gunier region(shaded green)
2. The Porod region(shaded yellow)
3. The Wide region(shaded red)
In the example above, data corresponding to lines 5-25 of the input file are selected to represent the Gunier region and will appear in the output when hitting the "Download" button. Likewise, lines 26-75 of the input files will represent the Porod region, and lines 76-90 of the Wide Region. Lines 1-9 and 91-100 will be excluded from the output
When the parameters are in "Input" mode, the slider bar will disappear and be replaced by input fields in the Control Area

Full: Zooms out to show the entire data range on the graph (default upon loading)
Gunier: Zooms in to the Gunier region, as defined by the initial default cutoffs and then by other user-defined controls, plus a few Porod region points
Porod: Zooms in to the Porod region, as defined by the initial default cutoffs and then by other user-defined controls, plus a few Gunier and Wide region points
Wide: Zooms in to the Wide region, as defined by the initial default cutoffs and then by other user-defined controls, plus a few Porod region points
Start: Zooms in to the first data points of your uploaded files
G-P: Zooms in to the interface between the Gunier and Porod regions of your data
P-W: Zooms in to the interface between the Porod and Wide regions of your data
End: Zooms in to the last data points of your uploaded files

Download: Downloads the user-adjusted file that represents averaged data across the three regions
Reset: Reloads the webpage for a new analysis

Slider: Use slider bars to update your dynamic graph:
1. The Gunier region
2. The Porod region
3. The Wide region
Input: Use input boxes or increment arrows to update your dynamic graph
- In "Input" mode, the top three input fields again determine how many image frames (input files) to include in your "Dynamic" output file for each region
- The bottom three input fields below adjust the three regions of the plot, similar to the slider bar that is below the graph when in "Slider" mode, where "Start" and "End" represent the boundaries for each representative section
- Again, the order for the set is Gunier, Porod, Wide

Shows the actual number of files processed to produce the graph and subsequent output
Shows the names of the files processed to produce the graph and subsequent output
The above should be checked in the event it appears that a file did not load properly or if SAXS curves appear strange (unintentionally mixed two or more datasets)

Click the link to download a .zip file consisting of the test files used in these instructions:

Unzip the directory before uploading files or the folder to the SAXS FrameSlice webapp